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4E11
4E11
規(guī)格:
貨期:
編號:B163768
品牌:Mingzhoubio

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產(chǎn)品名稱 4E11
商品貨號 B163768
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Storage Conditions liquid nitrogen vapor phase
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Derivation Animals were immunized with DYKDDDDK. Spleen cells were fused with NS-1 myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against a synthetic peptide Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (DYKDDDDK)
Cellular Products
immunoglobulin; monoclonal antibody; against a synthetic peptide Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (DYKDDDDK)
Tumorigenic Yes
Effects Forms ascites in pristane primed BALB/c mice.
Comments The antibody reaction with the peptide is calcium dependent and the antibody can be used to isolate fusion proteins tagged with the DYKDDDDK peptide. The resulting antigen - antibody complex can be dissociated by removal of Ca++ from the buffer.
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
Cryopreservation Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG2b
Name of Depositor Immunex Corporation
Deposited As mouse (B cell); mouse (myeloma)
U.S. Patent Number
References

Hopp TP, Prickett KS. Immunoaffinity purification system. US Patent 4,851,341 dated Jul 25 1989

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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