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CT6-1D7
CT6-1D7
規(guī)格:
貨期:
編號:B164298
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 CT6-1D7
商品貨號 B164298
Organism Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody recognizes the cytoplasmic region of both Fc gamma RI a1 and Fc gamma RI b2 in immunoadsorption assays and Western blot analysis but Fc gamma a1 is better recognized than Fc gamma b2.
Storage Conditions liquid nitrogen vapor phase
Derivation
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
Genes Expressed
immunoglobulin; monoclonal antibody; against the cytoplasmic tail of the human high affinity Fc receptor for IgG (Fc gamma RI)
Cellular Products
immunoglobulin; monoclonal antibody; against the cytoplasmic tail of the human high affinity Fc receptor for IgG (Fc gamma RI)
Comments
Animals were immunized with an 18-residue peptide in the cytoplasmic domain near the plasma membrane of FcgammaRIa1.
Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells.
The antibody recognizes the cytoplasmic region of both Fc gamma RI a1 and Fc gamma RI b2 in immunoadsorption assays and Western blot analysis but Fc gamma a1 is better recognized than Fc gamma b2.
Complete Growth Medium The base medium for this cell line is ATCC-formulated DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum 5% (final conc.)
  • NCTC 109 (InVitrogen Cat No. 21340-039) 5% (final conc.)
  • HL-1 Supplement (HL-1 medium supplement Lonza Cat No. 77227 or 77228) 1% (final conc.)
  • 0.1 mM hypoxanthine
  • 0.016 mM thymidine
Subculturing Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 2 x 105 and 8 x 105 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1; kappa light chain
Name of Depositor CL Anderson
Deposited As mouse (B cell); mouse (myeloma)
References

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

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