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HEL 92.1.7
HEL 92.1.7
規(guī)格:
貨期:
編號:B164590
品牌:Mingzhoubio

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產(chǎn)品名稱 HEL 92.1.7
商品貨號 B164590
Organism Homo sapiens, human
Tissue bone marrow
Cell Type erythroblast
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease erythroleukemia
Age 30 years
Gender male
Ethnicity Caucasian
Storage Conditions liquid nitrogen vapor phase
Clinical Data
30 years
Caucasian
male
Antigen Expression
Antigen expression: HLA A3, Aw32, Bw35; Ia+
Genes Expressed
hemoglobin; globin (G gamma, A gamma, epsilon, eta and alpha chains); beta-2-microglobulin; glycophorin
Comments
These cells differentiate spontaneously into erythroblast-like cells.
Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid).
Complete Growth Medium The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days
STR Profile
Amelogenin: X,Y
CSF1PO: 10
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 7
THO1: 7
TPOX: 11
vWA: 14,17
Name of Depositor PJ Martin
Deposited As Homo sapiens
References

Papayannopoulou T, et al. Human erythroleukemia cell line (HEL) undergoes a drastic macrophage- like shift with TPA. Blood 62: 832-845, 1983. PubMed: 6309287

Martin P, Papayannopoulou T. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science 216: 1233-1235, 1982. PubMed: 6177045

Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679

Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663

Riewald M, et al. Identification and characterization of the cytoplasmic antiproteinase (CAP) in human platelets. J. Biol. Chem. 271: 7160-7167, 1996. PubMed: 8636153

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