亚洲国产精品一区二区久久hs,精品国产一级在线观看,免 费 成人黄 色 大片,不卡中文一二三区,国产剧情麻豆mv在线观看,久久久久久久久久久精品,久久九九综合,久久久久久91香蕉国产

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > pPW121
▍最近瀏覽歷史
▍聯(lián)系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮(zhèn)海區(qū)莊市街道興莊路9號
  • 創(chuàng)e慧谷42號樓B幢401室
pPW121
pPW121
規(guī)格:
貨期:
編號:B234314
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 pPW121
商品貨號 B234314
Designations pPW121
Depositors F Puta
Biosafety Level 1
Vector Information
Size (kb): 4.3860001564025880
Vector: pPW121 (plasmid)
Promoters: Promoter lambda PR
Construction: pUN121
Marker(s):ampR,tetR
Construct size (kb): 4.386000156402588
Features: marker(s): ampR
marker(s): tetR
operator: lac
promoter: lambda PR
replicon: pMB1
repressor gene: cI
Applications
vector permitting positive selection for inserts
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--4.3; HindIII--4.3; BamHI--4.3.
Postive selection cloning vector which confers tetR (recommended concentration 10 ug/ml) only when an insert has been successfully cloned into the cI repressor gene.
Plasmid was derived from pUN121 by introduction of a BglII site into the cI gene. Plasmid pUN121 was derived from pTR262 by addition of EcoRI and SmaI/XmaI sites in the cI gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Puta F, Wambutt R. Construction of a new Escherichia coli-Saccharomyces cerevisiae shuttle plasmid cloning vector allowing positive selection for cloned fragments. Folia Microbiol. 37: 193-198, 1992. PubMed: 1505881

Nilsson B, et al. An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis. Nucleic Acids Res. 11: 8019-8030, 1983. PubMed: 6316281

Frantisek Puta, personal communication

Shipped freeze-dried
梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
灵武市| 合江县| 无为县| 五原县| 陕西省| 六安市| 榆社县| 泗洪县| 砀山县| 明星| 兴和县| 卓尼县| 昌宁县| 庐江县| 江达县| 韶山市| 如皋市| 会理县| 武安市| 仪征市| 北票市| 奉贤区| 抚远县| 塔城市| 泰宁县| 宜州市| 阜南县| 盖州市| 曲阳县| 浑源县| 民权县| 靖州| 响水县| 海晏县| 山丹县| 高陵县| 晴隆县| 莱阳市| 桦川县| 手机| 五峰|