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p2UGCAT
p2UGCAT
規(guī)格:
貨期:
編號:B236585
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱 p2UGCAT
商品貨號 B236585
Designations p2UGCAT
Depositors KR Yamamoto
Biosafety Level 1
Vector Information
Size (kb): 7.5999999046325680
Vector: p2UGCAT (plasmid)
Promoters: Promoter CYC1
Construction: p2UG, CAT
Marker(s):URA3,ampR
Construct size (kb): 7.599999904632568
Features: marker(s): ampR, URA3
promoter: CYC1
replicon: pMB1, 2 micron
enhancer: GRE
Applications
YE-type (episomal) shuttle vector
expression vector
reporter construct
shuttle vector
vector with sequences for protein export
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--7.6, EcoRI--2.2 (doublet), 1.7, 1.4; KpnI--7.6.
The URA3 marker is from S. cerevisiae but complements the ura4 allele from Sc. pombe.
Expression of chloramphenicol acetyltransferase is hormone-inducible in both S. cerevisiae and Sc. pombe hosts, in the presence of a glucocorticoid receptor such as that encoded by pG-N795 (ATCC 37813) or pART1/N795 (ATCC 37814).
Contains the CYC1 TATA region from S. cerevisiae (nt -178 to +49) fused at nt -178 to 3 copies of a 26 bp glucocorticoid response element (GRE) from the rat tyrosine aminotransferase gene.
A BglII/BamHI fragment with a chloramphenicol acetyltransferase coding region, its own AUG codon, and some SV40 sequences was cloned into the BamHI site of the polylinker. The other polylinker sites are 3' to this insert.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Picard D, et al. An inducible expression vector for both fission and budding yeast. Gene 86: 257-261, 1990. PubMed: 2108906

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梅經(jīng)理 17280875617 1438578920
胡經(jīng)理 13345964880 2438244627
周經(jīng)理 17757487661 1296385441
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